Coding

Part:BBa_K1218010:Design

Designed by: Alex Constantino   Group: iGEM13_Stanford-Brown   (2013-08-29)


Cas3 Endonuclease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1581
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 214
    Illegal SapI site found at 904


Design Notes

Contained 2 internal PstI sites. We used site-directed mutagenesis to remove the sites without altering the amino acid sequence.


Source

Amplified from DH5α genomic DNA.

References